Dr. Rashad R. Al-Hindi
King Abdulaziz University, Jeddah, Saudi Arabia
Title: Lytic kinetics of a cocktail of two Siphoviridae bacteriophages against Klebsiella pneumonia
Biography
Biography: Dr. Rashad R. Al-Hindi
Abstract
Exposure to particulate matter (PM) and lipopolysaccharide (LPS) triggers pulmonary diseases such as chronic obstructive pulmonary disease by inducing oxidative stress and chronic inflammatory response in lung tissues. Cordyceps militaris grown on germinated Rhynchosia nulubilis (GRC) has been reported to exert anti-inflammatory and antioxidant effects (ref). To improve the efficacy of GRC, we synthesized chitosan nanoparticles encapsulated with GRC (GCN). Our study aims to investigate the anti-inflammatory activity and the underlying mechanism of GCN in PM and LPS-stimulated RAW264.7 macrophages. Compared to RAW264.7 cells treated with PM and LPS, GCN significantly decreased the expression of PM and LPS-induced reactive oxygen species (ROS). GCN significantly also decreased the production of PM and LPS-induced nitric oxide (NO). The mRNA expression of PM and LPS-induced inflammatory mediators such as interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) was decreased in GCN-treated RAW 264.7 cells. The inhibitory effect of GCN on the expression of phosphorylated c-Jun, and phosphorylated NF-κB was observed in PM and LPS-stimulated RAW264.7 cells, which is associated with reduced inflammatory mediators. Conclusively, our results suggested that GCN might be a potent therapeutic agent against PM and LPS-induced inflammation by blocking oxidative stress and inflammatory reaction in macrophages.
